COMPARISION OF IN-VITRO ANTIDIABETIC SCREENING METHODS FOR CERTAIN SYNTHESIZED 4-PHENYL-6-SUBSTITUTED PHENYL-3,4-DIHYDRO PYRIMIDINE-2-ONE AND N-PHENYL-3-PHENYL-5- SUBSTITUTED PHENYL PYRAZOLINE ANALOGUES

A. Zechariah Jebakumar
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
Hassan S. Nondo
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
Siju K. George
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
G.Manoj
Dept of Pharmacology, C.L. Baid Metha College of Pharmacy, Thoraipakkam, Chennai - 600096, India

Antidiarrhoeal activity ,Randia uliginosa ,Traditional medicine ,Castor oil induced diarrhoea ,Enteropooling method ,

Two series of heterocyclic analogues were synthesized from substituted chalcones (C1-5) which was prepared by reaction of acetophenone (a) and aromatic aldehydes (b1-5). One series of N-phenyl pyrazoline derivatives (K1-K5) were synthesized by reaction of chalcones (C1-5) with phenyl hydrazine in acidic medium. Another series of 3,4-dihydropyrimidine-2-one derivatives (K6-K10) were synthesized by reaction of chalcones (C1-5) with ethanolic urea in alkali medium. The yield of the synthesized compounds ranged from 62-76%. The In-vitro antidiabetic screening was performed by non enzymatic glycosylation of haemoglobin assay, α-amylase enzyme inhibition assay, α-Glucosidase enzyme inhibition assay methods. The result of all heterocyclic analogues (K1-K10) indicates that have pronounced potency when compared to standard drugs. Among that analogues K1 & K6 showed nearly equal action and analogues K2 & K7 also showed appreciable activity when compared to the standard drugs at 50μg/ml, 100μg/ml, and 150μg/ml tested concentrations.

6 , 2 , 2016