IN VITRO SCREENING OF EFFECTIVE BIOCONTROL AGENTS AGAINST BEAN ANTHRACNOSE PATHOGEN, COLLETOTRICHUM LINDEMUTHIANUM

A. Zechariah Jebakumar
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
Hassan S. Nondo
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
Siju K. George
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
G.Manoj
Dept of Pharmacology, C.L. Baid Metha College of Pharmacy, Thoraipakkam, Chennai - 600096, India

Antidiarrhoeal activity ,Randia uliginosa ,Traditional medicine ,Castor oil induced diarrhoea ,Enteropooling method ,

Identification and selection of effective isolate of biocontrol agents is the first and foremost step in biological control. Biocontrol agents were selected based on their antagonistic activity against mycelial growth of the pathogen by following the dual culture technique. Evaluation of Trichoderma viride isolates against C. lindemuthianum growth under In vitro condition showed that all the isolates were effective in inhibiting the pathogen growth. Among the seven isolates (TVMGLT1, TV21GL, TVMNT7, T. harzianum, TV15, TVUV10 and TVMG5) tested, isolate TVMGLT1 recorded highest mycelial inhibition of 75.00% reduction over control and highest inhibition zone of 18.00mm. The increased antagonist overgrowth on the C. lindemuthianum was observed in TVMGLT1 (65.00mm) and TV21GL (63.00mm). Though all the isolates showed mycelial inhibition, the isolate TVMGLT1 recorded greater antagonistic activity against the growth of C. lindemuthianum. The bacterial antagonists like, Pseudomonas fluorescens and Bacillus subtilis isolates were evaluated against C. lindemuthianum growth. Among five Pseudomonas fluorescens isolates (PFSR, PF1, PFHS, PF8 and PFOOTY1) tested, PFSR showed higher inhibitory effect (33.33%) and highest inhibition zone of 20.00mm. Similarly, among four Bacillus subtilis isolates (BSG3, M2, CBE 4 and G1) tested, BSG3 recorded higher inhibition in mycelial growth (14.28%) and inhibition zone of 5.00mm.

4 , 1 , 2014