EVALUATION OF ANTIMICROBIAL ACTIVITY OF PUNICA GRANATUM PEEL EXTRACTS USING DIFFERENT SOLVENT SYSTEM
A. Zechariah Jebakumar
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
Hassan S. Nondo
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
Siju K. George
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
G.Manoj
Dept of Pharmacology, C.L. Baid Metha College of Pharmacy, Thoraipakkam, Chennai - 600096, India
Punica granatum are utilized by local people as the part of their meal. Peoples supposed to consume Punica granatum seed and their peel was thrown as waste. Present study was designed to evaluate the antibacterial activity of Punica granatum peel against human pathogens. The extract was prepared using water, ethanol, methanol, acetic acid and petroleum ether. Antimicrobial activity was tested against four gram positive bacteria and six gram negative bacteria. Evaluations were based on the zone of inhibition using Agar well diffusion assay. The inhibitory activity was found to be dose dependent. The maximum antimicrobial activity was reported at 10mg/ml dosage tested. Results showed that Punica granatum showed highly significant antimicrobial activity against both the classes of bacteria. Aqueous, ethanol and methanol extracts were found to be more active towards the microorganisms tested than acetic acid and petroleum ether extracts. However with respect to pathogens the effectiveness will vary depending on the nature of extraction as well as on concentration of extract being administered. Salmonella typhi and Proteus vulgaris was reported to have significant susceptibility against most of the extract. This study represents that aqueous, ethanol and methanol extracts of waste material (peel) of Punica granatum may be utilizes as a potential source of antimicrobial agents. Phytochemical analysis of P. granatum peel show presence of alkaloids, flavanoids, steroids, tannin, cardiac glycosides and terpenoids using different solvent system suggest extractions of bioactive compounds are solvent dependent.
4 , 1 , 2014
26 - 31