PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF FLOWER OF KIGELIA AFRICANA

A. Zechariah Jebakumar
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
Hassan S. Nondo
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
Siju K. George
Dept. of Research and Scientific studies, Prince Sultan Military college of Health Sciences, Dhahran-31932, Kingdom of Saudi Arabia.
G.Manoj
Dept of Pharmacology, C.L. Baid Metha College of Pharmacy, Thoraipakkam, Chennai - 600096, India

Antidiarrhoeal activity ,Randia uliginosa ,Traditional medicine ,Castor oil induced diarrhoea ,Enteropooling method ,

Antioxidant activity of methanolic extract of flower of Kigelia Africana was evaluated using the free radical scavenging activity of the Hydrogen peroxide radical scavenging activity, Reducing power assay and Phosphomolybdenum method. The phytochemical studies on the methanolic extracts of Kigelia africana have revealed the presence of phenolic compounds like Flavonoids and and terpinoids. In the method H2O2 scavenging activity of hydrogen peroxide method the absorbance values of Kigelia africana extracts shows lower activity with that of standard values. Here ascorbic acid is used as standard. In this reducing power method the absorbance values of Kigelia africana show lower activity with that of standard values. Here ascorbic acid is used as standard. In this phosphomolybdenum method increase in the reaction mixture indicates increasing reducing power. For Kigelia africana extracts the absorbance values equal at 40 μg and from 20μg, 60 μg, 80 μg and 100 μg it shows this is having decreasing reaction. So Kigelia africana extract show comparable activity at 40 μg. Here DMSO is used as standard drug. The methanol extracts of flower of Kigelia africana was exhibited less potent anti-oxidant activity in H2O2 scavenging hydrogen peroxide method and reducing power method and potent anti-oxidant activity in Phosphomolybdenum method.

6 , 1 , 2016